HPLC Monitoring of Malondialdehyde in Patients with Endometrial and Ovarial Cancer

Authors

  • Jana Matějčková Department of Biochemistry, Molecular and Cell Biology, Third Faculty of Medicine, Charles University, Prague
  • Martin Samec Department of Gynaecology and Obstetrics, Faculty Hospital Královské Vinohrady, Prague
  • Martin Jaček Department of Biochemistry, Molecular and Cell Biology, Third Faculty of Medicine, Charles University, Prague
  • Petr Tůma Department of Biochemistry, Molecular and Cell Biology, Third Faculty of Medicine, Charles University, Prague

Keywords:

malondialdehyde, HPLC, derivatization, oxidative stress, carcinoma

Abstract

A method for determination of malondialdehyde (MDA) as the major biomarker of oxidative damage of cellular membranes was developed. MDA was transformed to its 2,4-dinitrophenylhydrazone, which was then separated by HPLC. The LOD and repeatability of the method is satisfactory for monitoring MDA in blood plasma. The performed clinical study revealed systematically enhanced MDA levels in patients with gynaecological carcinoma in comparison with the control group.

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Published

2011-05-15

How to Cite

Matějčková, J., Samec, M., Jaček, M., & Tůma, P. (2011). HPLC Monitoring of Malondialdehyde in Patients with Endometrial and Ovarial Cancer. Chemické Listy, 105(5), 375–380. Retrieved from http://www-.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/1143

Issue

Vol. 105 No. 5 (2011)

Section

Articles